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By Alton Meister

Advances in Enzymology and similar components of Molecular Biology is a seminal sequence within the box of biochemistry, providing researchers entry to authoritative stories of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, supplying an unmatched view of the historic improvement of enzymology. The sequence bargains researchers the newest figuring out of enzymes, their mechanisms, reactions and evolution, roles in complicated organic strategy, and their program in either the laboratory and undefined. every one quantity within the sequence good points contributions by means of major pioneers and investigators within the box from around the globe. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.

With its wide variety of subject matters and lengthy ancient pedigree, Advances in Enzymology and comparable parts of Molecular Biology can be utilized not just via scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition by way of any scientist drawn to the invention of an enzyme, its homes, and its purposes.

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J . , 108,37 (1949). 28. Muralt, A. von, Die Signalubermittlur~gi m Nerven. Birkhauser, Basel, 1946. 29. Pryor, M. G. , Progress in Biophysics. Academic Press, Vol. 1, Chapt. 9, 1950. 30. Astbury, W. , Proc. Roil. London, B134, 303 (1947). 31. Meyer, K. , and Picken, L. E. , I’roc. Roy. London, B124,29 (1937). , Ann. N . Acad. , 47,715 (1947). Hill, A. , and others, Proc. Roy. London, B137, 40 (1950). Hill, A. , Biochim. el Biophys. Acta, 4, 4 (1950). Fruton, J. , and others, Ann. N . Acad. , 45,357 (1944).

There are three ways in which the validity of the assumption of a separate phase may be tested experimentally. They are: (I) analysis; (2) electrical measurements; (3) kinetic studies; and (4)use of dyes to determine pH changes at a surface. (1) Analysis. Hanson, Neale, and Stringfellow (30) suggested REACTIONS AT INTERFACES 43 that the adsorption of dyes on cellulose could be treated by the Donnan method. They found that their theoretical results were very closely followed by the analytical figures, though Neale and Stringfellow (47) in their study of oxycelluloses could not obtain constant dissociation values for the carboxyl groups, using the Donnan theory.

P-Nitrophenol was used to determine the bulk pH of the solution. Preliminary experiments showed aggreement t o a few millivolts between the values of E thus calculated and from electrokinetic observations on cetane sulfonates. 01 N NaCl solution. The values of pH thus obtained agree well with those calculated by applying the Donnan equations to the surface phase surrounding the ovalbumin molecule (Fig. 1). Daniclli could have expressed his results as potentials, which would of course have resulted in close correspondence between the Donnan and electrokinetic potentials a t the surface of the protein.

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